Table 2.

Effect of CYP administration on passive and active electrical properties of bladder sensory neurons from WT and Asic3 KO mice

	WT				Asic3 KO
	Saline		CYP		Saline		CYP
	TTX-R	TTX-S	TTX-R	TTX-S	TTX-R	TTX-S	TTX-R	TTX-S
Membrane capacitance, pF	31 ± 2	42 ± 3	30 ± 2	37 ± 5	30 ± 2	40 ± 5	27 ± 2	33 ± 2
Resting membrane potential, mV	−55 ± 2	−60 ± 2	−54 ± 1	−50 ± 2d	−56 ± 2	−57 ± 3	−53 ± 2	−50 ± 1f
AP rheobase, pA	484 ± 66	587 ± 91	419 ± 90	157 ± 73e	500 ± 121	596 ± 107	141 ± 31b	134 ± 42d
AP threshold, mV	−26 ± 1	−23 ± 1	−27 ± 1	−26 ± 1c	−25 ± 1	−23 ± 1	−30 ± 1b	−28 ± 1c
AP duration, ms	3.8 ± 0.3	2.8 ± 0.3	3.8 ± 0.5	3.5 ± 0.5	3.8 ± 0.4	2.5 ± 0.4	2.7 ± 0.2	3.1 ± 0.6
AP amplitude, mV	98 ± 3	100 ± 5	97 ± 3	91 ± 5	101 ± 5	97 ± 6	95 ± 4	85 ± 5
Magnitude of afterhyperpolarization, mV	−15 ± 2	−7 ± 2	−16 ± 1	−18 ± 3c	−13 ± 3	−11 ± 3	−23 ± 2a	−22 ± 3

Values are means ± SE; n = 10–22. Wild-type (WT) and acid-sensing ion channel subunit 3 (Asic3) knockout (KO) mice received 3 doses of saline or cyclophosphamide (CYP), and sensory neurons were isolated a day after the last dose as described in materials and methods. Neurons were classified based on the sensitivity of the action potential (AP) to tetrodotoxin (TTX) as TTX sensitive (TTX-S) or TTX resistant (TTX-R). APs were evoked by 4-ms current pulses. Statistically significant: ^aP < 0.05, Asic3 KO CYP vs. WT CYP or Asic3 KO saline; ^bP < 0.001, Asic3 KO CYP vs. WT CYP or Asic3 KO saline; ^cP < 0.05, WT saline vs. WT CYP or Asic3 KO saline vs. Asic3 KO CYP; ^dP < 0.01, WT saline vs. WT CYP or Asic3 KO saline vs. Asic3 KO CYP; ^eP < 0.001, WT saline vs. WT CYP; ^fP < 0.01, Asic3 KO saline vs. Asic3 KO CYP (Kruskal-Wallis test followed by Dunn’s multiple comparisons test).