Skip to main content
. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Am J Transplant. 2021 Oct 12;22(2):427–437. doi: 10.1111/ajt.16852

Figure 3. IL-6 deficiency decreases Th17-lineage frequency and absolute numbers in hyperlipidemic mice.

Figure 3.

Groups of age matched ApoE+/+IL-17cre/tdTomato IL-6+/+, ApoE−/−IL-17cre/tdTomato IL-6+/+ and ApoE−/−IL-17cre/tdTomato IL-6−/− mice were maintained on normal chow or high fat diet for a minimum of four weeks. Splenocytes were isolated and stained with antibodies specific for murine CD3 and CD4. Cells were examined for expression of CD3, CD4 and tdTomato by flow cytometry. (A) Representative flow plots gated on live CD3+CD4+ splenocytes showing the percentage of tdTomato+ cells. (B) Combined frequencies of all CD3+ splenocytes expressing tdTomato. (C) Absolute number of all CD3+ splenocytes expressing tdTomato. Shown is the combined mean. (D) Combined frequencies of CD3+CD4+ splenocytes expressing tdTomato. (E) Absolute number of CD3+CD4+ splenocytes expressing tdTomato. Shown is the combined mean of ≥5 mice per group. Significance was determined using unpaired Students t-Test. Error bars represent standard deviation. *** = P < 0.0005, ** = P < 0.005, * = P < 0.05.