KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| Escherichia coli | CGC | OP50 |
| Chemicals, peptides, and recombinant proteins | ||
| 1-octanol | Sigma-Aldrich | Cat#112615 |
| Glycerol | Sigma-Aldrich | Cat#G5516 |
| Isoamyl alcohol | Sigma-Aldrich | Cat#AX1440 |
| Na-acetate | Sigma-Aldrich | Cat#S2889 |
| Diacetyl | Sigma-Aldrich | Cat#B0682 |
| Ethyl alcohol | Pharmco | Cat#111000200 |
| Chloroform | Sigma-Aldrich | Cat#C2432 |
| Dimethyl sulfoxide | Sigma-Aldrich | Cat#D5879 |
| Bicuculline | VWR | Cat#TBC1890 |
| Forskolin | VWR | Cat#102987-308 |
| Gluture | Fisher Scientific | Cat#NC0632797 |
| Experimental models: Organisms/strains | ||
| N2 | CGC (Brenner, 1974) | N2 |
| clh-1(syb2417) | This study (Sunybiotech) | PHX-2417 |
| clh-1(syb2417); blcEx476[pT02B11.3::GFP1-10; pSra-6::RFP] | This study | BLC453 |
| deg-1(u38u421) | CGC (Hart et al., 1999) | TU38 |
| trpa-1(ok999) | CGC (Consortium, 2012) | RB1052 |
| clh-1(ok658) | CGC (Consortium, 2012) | RB833 |
| blcEx319[pT02B11.3::pGM87; pT02B11.3::clh-1 RNAi; Punc-122::GFP] | This study | BLC315 |
| clh-1(ok658); blcEx410[pT02B11.3::clh-1(cDNA); pT02B11.3::pGM87; Punc-122::GFP] | (Grant et al., 2015) | BLC303 |
| clh-1(ok658); blcEx473[pSra-6::clh-1 (cDNA); pSra-6::GCaMP-6s] | This study | BLC447 |
| osm-9 (ky10) | CGC (Colbert and Bargmann, 1995) | CX10 |
| tax-2 (p691) | CGC (Komatsu et al., 1996) | PR691 |
| kyIs39[sra-6::GFP + lin-15(+)] | CGC (Troemel et al., 1995) | CX3465 |
| clh-1(ok658); kyIs39 [psra-6::GFP + lin-15(+)] | This study | BLC319 |
| odr-3 (n2150) | CGC (Colbert and Bargmann, 1995) | CX2205 |
| blcEx446[Psra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC401 |
| clh-1(ok658); blcEx446[pSra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC454 |
| clh-1(ok658); blcEx477[pT02B11.3::clh-1 (cDNA); pSra-6::GCaMP-6s] | This study | BLC455 |
| deg-1(u38u421); blcEx469[pSra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC441 |
| trpa-1(ok999); blcEx478[pSra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC456 |
| blcEx447[pT02B11.3::GCaMP-6s; Punc-122::GFP] | This study | BLC402 |
| clh-1(ok658); blcEx447[pT02B11.3::GCaMP-6s; Punc-122::GFP] | This study | BLC469 |
| lite-1(ce314); unc-25(e156); kanls6[Pvap-1::frt2::CoChR::sl2e::TagRFP; pT02B11.3::Flp; pSra-6::GCaMP5.0; Plin44::mCherry] | (Duan et al., 2020) | ST2499 |
| kanIs6; lite-1(ce314); kanEx644[Psra-6::lgc-38 RNAi ; Punc-122::GFP] | (Duan et al., 2020) | ST2181 |
| clh-3(ok763) | CGC (Consortium, 2012) | RB900 |
| clh-3(ok763); clh-1(ok658) | This study | BLC375 |
| clh-1(ok658); Ex[pT02B11.3::clh-3b; Punc-122::GFP] | This study | BLC448 |
| clh-1(ok658); blcEx474[pT02B11.3::rCLC-2 (cDNA); pT02B11.3::pGM87] | This study | BLC328 |
| blcEx53[pT02B11.3::pGM87] | (Grant et al., 2015) | BLC44 |
| clh-1(ok658); blcEx53[pT02B11.3::pGM87] | (Grant et al., 2015) | BLC295 |
| pde-1(nj57); pde-5(nj49); pde-3(nj59); pde-2(tm3098) | CGC (Liu et al., 2010) | TQ1828 |
| pde-1(nj57); pde-5(nj49); pde-3(nj59); pde-2(tm3098); blcEx402[pT02B11.3::clh-1 RNAi; Punc-122::GFP] | This study | BLC371 |
| pde-4(ce268) | CGC (Charlie et al., 2006) | KG744 |
| pde-4(ce268) II; blcEx421[PT02B11.3::clh-1 RNAi; Punc-122::GFP] | This study | BLC449 |
| pde-4(ce268); clh-1(ok658); blcEx446[pSra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC450 |
| pde-4(ce268); deg-1(u38u421) | This study | BLC444 |
| acy-1(md1756) | CGC (Schade et al., 2005) | KG522 |
| clh-1(ok658); acy-1(md1756); blcEx446[pSra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC451 |
| acy-1(md1756); deg-1(u38u421) | This study | BLC435 |
| nlp-3(ok2688) | CGC (Consortium, 2012) | RB2030 |
| pde-4(ce268); blcEx446[pSra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC457 |
| acy-1(md1756); blcEx446[pSra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC458 |
| blcEx361[pT02B11.3::twk-33 RNAi; Pvap-1::RFP] | This study | BLC338 |
| clh-1(ok658); blcEx479[Pvap-1::clh-1 (cDNA); Punc-122::GFP] | This study | BLC496 |
| clh-1(ok658); blcEx480[Psra-6::clh-1 RNAi; Punc-122::GFP] | This study | BLC497 |
| blcEx481[pT02B11.3::Superclomeleon] | This study | BLC498 |
| clh-1(ok658); blcEx481[pT02B11.3::Superclomeleon] | This study | BLC499 |
| blcEx482[pT02B11.3::unc-25 RNAi; Psra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC500 |
| clh-1(ok658); blcEx483[Psra-6::lgc-38 RNAi; Psra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC501 |
| blcEx484[Psra-6::pde-4; Punc-122:GFP] | This study | BLC502 |
| blcEx485[Psra-6::nlp-3 RNAi; Punc-122::GFP] | This study | BLC503 |
| clh-1(ok658); blcEx486[Psra-6::eat-4 RNAi; Punc-122:GFP] | This study | BLC504 |
| blcEx487[Psra-6::eat-4; Punc-122::GFP] | This study | BLC505 |
| clh-1(ok658); blcEx488[Pnpr-9::glr-5 RNAi; Punc-122::GFP] | This study | BLC506 |
| kcc-3(ok228) | CGC (Consortium, 2012) | RB688 |
| clh-1(ok658); blcEx482[pT02B11.3::unc-25 RNAi; Psra-6::GCaMP-6s; Punc-122::GFP] | This study | BLC507 |
| Oligonucleotides | ||
| pPD95_75 | A gift from Andrew Fire | RRID:Addgene_1494 |
| pPD_Pnpr-4::G-CaMP6s | (Hori et al., 2018) | RRID:Addgene_117423 |
| pSH87(Pflp-13::gfp1-10) | (He et al., 2019) | RRID:Addgene_127705 |
| Additional oligonucleotides | Table S2 | N/A |
| Software and algorithms | ||
| Fiji (ImageJ) | NIH |
http://fiji.sc RRID:SCR_002285 |
| Micro-manager | Vale Lab, UCSF |
https://micro-manager.org/ RRID:SCR_000415 |
| Clampfit | Molecular Devices |
http://www.moleculardevices.com/products/software/pclamp.html RRID:SCR_011323 |
| GraphPad Prism | GraphPad software |
http://www.graphpad.com/ RRID:SCR_002798 |
| Biorender | BioRender |
http://biorender.com RRID:SCR_018361 |