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. 2022 Feb 3;12:1911. doi: 10.1038/s41598-022-05841-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cyclic imaging with the MACSima Imaging Platform. (a) MACSima System with fully automated robotic liquid handling and image acquisition. (b) Erasure techniques based either on photobleaching of the dye, or disruption of the labeling conjugate by release reagent. The release reagent leads to a rapid detachment of the fluorescent dye only (REAdye_lease Antibodies) or disruption of the labeling conjugate with a spontaneous dissociation of the monomerized antibody fragments and the fluorescent dye (REAlease Antibodies) from their target epitopes. (c) Cyclic imaging. (d) Image analysis with the MACS iQ View Software consisting of cellular segmentation, clustering, and visualization of clustered cells across the original image.