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. 2022 Feb 3;12:1848. doi: 10.1038/s41598-022-05947-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of WDR5 degradation on chromatin-associated H3K4me3. (a) Venn diagram, displaying the overlap of H3K4me3 ChIP-Seq peaks in non-treated (NT) or treated (18 h/100 μM IAA) AIDW cells. N = 3 for each set. (b) Heatmaps of the average normalized peak intensity (100 bp bins) for H3K4me3 peaks in NT and IAA-treated AIDW cells. Peaks are ranked according to the NT sample. Included are regions 3 kb upstream (− 3) and downstream (+ 3) of the peak zenith (0). (c) Volcano plot, comparing the log2FC in H3K4me3 peak intensity (IAA versus NT) against the − log10(adjusted p-value). (d) Averaged H3K4me3 peak shape and distribution in NT and IAA-treated AIDW cells, relative to the transcription start site (TSS), the transcription end site (TES), and 1 kb upstream and downstream of each. (e) Venn diagram, displaying the relationship between genes with decreased H3K4me3 and transcripts displaying significant decreases or increases in IAA-treated versus NT cells. (f) Graph showing the percentage of genes with not changed (NC; gray) or decreased (DN; red) H3K4me3 (me3) levels in IAA-treated cells, binned according to whether transcripts for those genes are increased (UP), decreased (DN), or not changed (NC) by IAA-treatment in RNA-Seq. p-values for UP against NC and DN against NC are shown; the p-value for UP against DN is 0.1738. (g) Scatter plots comparing log2FC in H3K4me3 versus RNA for IAA-treated AIDW cells. Comparisons are for (left) all genes that have a measurable H3K4me3 peak and a measurable transcript, and (right) genes that are bound by WDR5 in Ramos cells⁴ and have measurable H3K4me3 and RNA signals. The coefficient of determination (R²) is shown inside each plot.