Skip to main content
. 2022 Jan 14;54(1):47–60. doi: 10.1038/s12276-021-00663-2

Fig. 6. NEDD4 promotes bladder cell viability and migratory ability via the NRF2/KLF8/miR-132 axis.

Fig. 6

a RT-qPCR and western blot assay was used to examine the silencing efficiency of siNRF2-1 and siNRF2-2. b RT-qPCR was used to examine the expression of NEDD4, KLF8, miR-132, and NRF2 in response to oe-NEDD4 and siNRF2. c Western blot assay was used to examine the expression of NEDD4, KLF8, and NRF2 normalized to GAPDH in response to oe-NEDD4 and siNRF2. d MTT assay was used to examine cell viability in response to oe-NEDD4 and siNRF2. e Flow cytometry was used to examine apoptosis in response to oe-NEDD4 and siNRF2 under etoposide induction. f Transwell assay was used to examine cell migration in response to oe-NEDD4 and siNRF2 (×200). g Western blot assay was used to examine expression of the apoptosis-related proteins Bax and BCl-2, tumor suppressor proteins p53 and p21, and metastasis-related proteins vimentin, N-cadherin, and E-cadherin normalized to GAPDH in response to oe-NEDD4 and siNRF2. *P < 0.05 versus the si-NC or oe-NC + si-NC group; #P < 0.05 versus the oe-NEDD4 + si-NC group. The experimental results are the measurement data and are expressed as the mean ± standard deviation. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. Statistical analysis in relation to time-based measurements within each group was performed using repeated-measures ANOVA, followed by Bonferroni’s post hoc test. Cell experiments were independently repeated three times.