Fig. 7. NEDD4 facilitates tumorigenesis of bladder cancer by mediating the KLF8/miR-132/NRF2 axis in vivo.

a Images of xenograft tumors from nude mice. b Quantification of tumor volume in mice in response to NEDD4 overexpression and NRF2 silencing. c Quantification of tumor weight in mice in response to NEDD4 overexpression and NRF2 silencing. d RT-qPCR was used to examine the expression of NEDD4, KLF8, miR-132, and NRF2 in tumors in response to NEDD4 overexpression and NRF2 silencing. e Western blot assay was used to examine expression of NEDD4, KLF8, miR-132, and NRF2 in tumors normalized to GAPDH in response to NEDD4 overexpression and NRF2 silencing. f H&E staining (×200) of mouse lung metastatic nodules in response to NEDD4 overexpression and NRF2 silencing. g The number of lung metastatic nodules in response to NEDD4 overexpression and NRF2 silencing. *P < 0.05 versus the vector + sh-Ctrl group; ^#P < 0.05 versus the NEDD4 + sh-Ctrl group. The experimental results are the measurement data and are expressed as the mean ± standard deviation. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. Statistical analysis in relation to time-based measurements within each group was performed using repeated-measures ANOVA, followed by Bonferroni’s post hoc test. n = 10.