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. 2022 Feb 3;12:1828. doi: 10.1038/s41598-022-05491-6

Table 1.

Egg composition and yolk testosterone concentration.

	Experiment 1
Variable					Overall P-value
	EW	YW	AW	YT	EW	YW	AW	YT
	Egg weight (g)	Yolk weight (%)	Albumin weight (%)	Yolk testosterone (ng/g)	EW	YW	AW	YT
Maternal age					< 0.0001	< 0.0001	< 0.0001	0.0260
Young	56.9 ± 0.20^c	24.7 ± 0.12^b	63.5 ± 0.15^a	1.46 ± 0.14^a
Ideal	61.5 ± 0.28^b	28.5 ± 0.15^a	59.9 ± 0.14^c	1.31 ± 0.03^ab
Old	64.2 ± 0.34^a	28.4 ± 0.16^a	60.35 ± 0.16^b	1.06 ± 0.07^b
Maternal treatment					< 0.0001	< 0.0001	< 0.0001	0.7605
Trt 1 (AV × AV)	61.33 ± 0.61^a	26.44 ± 0.30^b	62.13 ± 0.29^a	1.39 ± 0.07
Trt 2 (CC × CC)	60.55 ± 0.88^b	27.65 ± 0.47^a	60.62 ± 0.40^b	0.21 ± 0.17
Trt 3 (AV × CC)	60.92 ± 0.87^ab	27.55 ± 0.48^a	60.85 ± 0.41^b	1.25 ± 0.19
Trt 4 (CC × FC)	60.31 ± 0.73^b	27.66 ± 0.45^a	60.94 ± 0.42^b	1.34 ± 0.13
Trt 5 (AV × FC)	60.66 ± 0.71^ab	27.55 ± 0.47^a	60.86 ± 0.44^b	1.20 ± 0.08
Materal age × maternal Trt					0.4483	0.4483	0.5200	0.9677

LSMean ± SEM for egg quality traits, egg composition and yolk testosterone of eggs from hens in Experiment 1 collected at three different ages.

^abcValues within a column for Maternal Age and Maternal Rearing × Adult Housing treatments with different superscripts differ P < 0.05.