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. 2022 Feb 3;13(2):107. doi: 10.1038/s41419-022-04560-y

Fig. 4. miR-582 overexpression inhibits proliferation and promotes survival of pre-B cells in vitro.

Fig. 4

A, B Total pre-B, large pre-B and small pre-B cells were infected with pre-miR-582 or anti-miR-582 lentivirus or their controls, and cultured for 5 days. Cell proliferation was determined using the CFSE labeling assay (n = 3). C, D Total pre-B, large pre-B, and small pre-B cells were harvested from WT and miR-582 KO mice and cultured for 72 h in vitro. Cell proliferation was determined using the CFSE labeling assay (n = 3). E, F pre-B cells were infected with pre-miR-582 or anti-miR-582 lentivirus or their controls, and cultured for 72 h. Apoptosis was evaluated by FACS after staining for Annexin V and 7-AAD. The percentages of apoptotic (Annexin V+/7-AAD−/+) pre-B cells were quantitatively compared (n = 5–6). G, H Total pre-B, large pre-B, and small pre-B cells were harvested from WT and miR-582 KO mice and cultured for 72 h in vitro. Apoptotic cells were determined by flow cytometry after Annexin V and 7-AAD staining and quantitatively compared (n = 3). Bars represent means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ns P > 0.05.