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. 2022 Feb 3;13(2):107. doi: 10.1038/s41419-022-04560-y

Fig. 7. miR-582 simultaneously targets mTORC2/AKT signaling to attenuate pre-B cell proliferation and survival.

Fig. 7

A Pre-B cells were infected with anti-miR-582 lentivirus and cultured for 72 h. The level of p-AKT, AKT, p-FoxO1, and FoxO1 was determined by Western blotting with β-actin as a reference control (n = 4–5). B Pre-B cells were infected with anti-miR-582 lentivirus and cultured in the presence of the mTORC2 inhibitor JR-AB2-011 for 72 h. The level of p-AKT, AKT, p-FoxO1, and FoxO1 was determined by Western blotting with β-actin as a reference control (n = 4). C Pre-B cells were infected with anti-miR-582 lentivirus and cultured in the presence of the mTORC2 inhibitor JR-AB2-011 for 72 h. Cell proliferation and apoptosis were evaluated by CFSE labeling and Annexin V+ and 7-AAD+, respectively, followed by flow cytometry, and quantitatively compared (n = 3). D The signaling pathway of miR-582 regulate the proliferation and survival of pre-B cell. Bars represent means ± SEM, *P < 0.05, **P < 0.01.