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. 2022 Feb 3;13:651. doi: 10.1038/s41467-022-28272-1

Fig. 3. MitoMISS longevity depends on ATFS-1 and is independent of UPRmt-induced chaperones.

Fig. 3

a, b Expression levels of canonical UPRmt specific-transcriptional reporters hsp-60 (a) and hsp-6 (b) were monitored upon inhibition of mitochondrial protein import components, timm-23, tomm-40, timm-22 and gop-3. n = 3 biologically independent experiments with at least 20 worms per condition. One-way ANOVA with Dunnett’s multiple comparison test. Exact sample size and P values are included in the Source Data file. c, d Expression levels of hsp-60 (c) and hsp-6 (d) upon MitoMISS and in the absence of ATFS-1 or DVE-1. n = 3 biologically independent experiments with at least 20 worms per condition. One-way ANOVA with Tukey’s multiple comparison test. Exact sample size and P values are included in the Source Data file. a.u. arbitrary units. e, f Lifespan curves upon MitoMISS in the absence and presence of ATFS-1 (e) and DVE-1 (f). g Lifespan curves upon MitoMISS in the absence and presence of HSP-60. n = 2 biologically independent experiments. Lifespan curves were statistically analyzed with the Log-rank (Mantel–Cox) test; detailed values are shown in Supplementary Table 2. (**** denotes P < 0.0001, *** denotes P < 0.001,** denotes P < 0.01 and * denotes P < 0.05).