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. 2022 Feb 3;13(2):109. doi: 10.1038/s41419-022-04542-0

Fig. 2. LINC01123 regulates the expression of B7–H3 through sponging miR-214-3P.

Fig. 2

A Plasmids containing wild-type (WT) or mutant (MUT) 3′UTR of LINC01123 and B7–H3 sequences were constructed. B Dual-luciferase-reporter gene assay was performed to evaluate the direct interactions between LINC01123, B7–H3, and miR-214-3P. C Effect of LINC01123 on miR-214-3P expression, as detected by qRT-PCR. D Effect of miR-214-3P on B7–H3 expression, as detected by qRT-PCR. E Effect of LINC01123 on B7–H3 expression, as detected by qRT-PCR. F Western blotting was used to detect the impact of LINC01123 on B7–H3 expression. G Western blotting was used to detect the effects of miR-214-3P on B7–H3 expression. *P < 0.05 compared with cells without treatment. WT wild-type sequences were constructed into the PmirGlo vector group, MUT mutant sequences were constructed into the PmirGlo vector group, PC positive control group, PmirGlo empty plasmid group, NC normal control, OE over-expression. The experiment was repeated three times.