Fig. 4. Upregulation of LINC01123 or B7–H3 or downregulation of miR-214-3p induces dysfunction of CD8⁺T cells.

CD8⁺T cells were isolated from PBMCs using immunomagnetic beads. After activation and amplification, CD8⁺T cells were cocultured with different CAL-27 cell treatment groups. A Expression of TNF-α, IFN-γ, perforin, and granzyme B in CD8⁺T-cell population was analyzed with flow cytometry. B Statistical analysis of the results from flow cytometry of CD8⁺T cells. C Expression levels of TNF-α, IFN-γ, perforin, and granzyme B in CAL-27–CD8⁺T cocultured supernatants, as detected by ELISA. D CCK8 cytotoxicity test of CD8⁺T cells. E ELISA was used to assess the immune activity of CD8⁺T cells after recombinant human B7–H3 treatment. *P < 0.05 compared with cells without treatment. The experiment was repeated three times.