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. 2022 Feb 3;13(2):109. doi: 10.1038/s41419-022-04542-0

Fig. 6. The function of LINC01123 in HNSCC can be reversed by miR-214-3p silencing. HNSCC cells were transfected with sh-LINC01123 in the presence of inhibitor–NC or miR-214-3p inhibitor.

Fig. 6

A Relative expression levels of LINC01123, miR-214-3p, and B7–H3 were determined by qRT-PCR. B B7–H3 protein expression was determined by western blot analysis. C CAL-27 cell viability was measured using a CCK8 assay. D Migration of CAL-27 cells was determined by scratch wound-healing assay. E Statistical results of the scratch-healing test. F Migration and invasion of CAL-27 cells, as determined by transwell migration and invasion assay. G Number of migration and invasion cells. H Apoptosis rate and cell-cycle stage were detected by flow cytometry. I Statistical analysis of apoptosis results. J Statistical analysis of cell cycle stage results. K CD8+T cells were cocultured with CAL-27 cells that had been transfected with plasmids, and the expression of TNF-α, IFN-γ, perforin, and granzyme B in the CD8+T cells was analyzed by flow cytometry. L Statistical analysis of CD8+T-cell flow cytometry results. M After different treatments, CAL-27 cells were cocultured with CD8+T cells, and the expression levels of TNF-α, IFN-γ, perforin, and granzyme B in coculture supernatants were detected by ELISA. N Killing effect of CD8+T cells on CAL-27 cells, as assessed by CCK8. O Tumor-volume growth curves of the sh-LINC01123 + inhibitor–NC group and sh-LINC01123 + miR-214-3p-inhibitor group. P Tumor weights of tumor-bearing C3H mice. Q Tumor images at week 4. R Immunofluorescence staining for CD4 and CD8 in tumor specimens (×400). S Statistical analysis of CD4 and CD8 immunofluorescence results. T. Expression of TNF-α, IFN-γ, perforin, and granzyme B in peripheral blood of tumor-bearing C3H mice, as detected by ELISA. *P < 0.05 versus the sh-LINC01123 + inhibitor–NC group. The experiment was repeated three times.