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. 2022 Feb 3;13(2):109. doi: 10.1038/s41419-022-04542-0

Table 1.

The list of primers and probes.

Gene Primer Sequence(5′−3′)
Primers for qRT-PCR
LINC01123 Forward CAGCAGCAGCAGTGAGGAAGC
Reverse CGACCGAGGTGACAACGATGAC
miR-214-3p Forward GCGACAGCAGGCACAGACA
Reverse AGTGCAGGGTCCGAGGTATT
B7–H3 Forward CTGACAGATACCAAACAGCTGG
Reverse CGAAATCCCGGATGCTCA
U6 Forward CTCGCTTCGGCAGCACA
Reverse CTCAACTGGTGTCGTGGA
β-actin Forward TGACGTGGACATCCGCAAAG
Reverse CTGGAAGGTGGACAGCGAGG
Primers for stem-loop RT-PCR of miRNAs
U6 RT Primer AACGCTTCACGAATTTGCGT
miR-214-3p RT Primer GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTGCC
Sequences of probes for RNA FISH
LINC01123 5’-DIG- CCCTTGCATGGTACACTTGAAATAGATGGGCTGGA-DIG-3’
sh-RNAs target sequence
sh-B7–H3 Homo sapiens GCAGCTGACAGATACCAAACA
sh-B7–H3 mouse GGAAGTCCAGGTCTCTGAAGA