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. 2022 Feb 3;12:1885. doi: 10.1038/s41598-022-05640-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation of BRs from S1 Ig assays using DBS eluates and Hybrid DABA assay using serum. Serum samples and paired DBS eluates from 439 sero-positive and 382 sero-negative individuals were assayed using hybrid DABA and the S1 IgG and IgM capture assays, respectively. BRs from DABA serum assay were plotted against those generated from the DBS eluate on a Log10 scale. Samples from sero-positive individuals are shown with filled circles and those from sero-negative individuals with empty circles. Correlation of the DBS and serum BRs with each assay is shown graphically along with the coefficient of correlation, r, and the significance, p, generated using PRISM software. The dotted lines represent the cut-off for each assay. Discordant samples, namely those that were DABA-positive and DBS-negative (n = 9) are represented by a square and samples which were DABA-negative and DBS-positive (n = 3) represented by a triangle.