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. 2022 Feb 3;12:1885. doi: 10.1038/s41598-022-05640-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reactivity of eluates on IgG S1 from full and partial DBS samples. DBS samples from 20 different patients were extracted as full, half and quarter spots as described in the methods. The reactivity of the three resulting eluates from each individual was tested in the IgG S1capture. The resulting reactivity (BRs) of the full, half and quarter spot eluates is shown for each individual, each line representing a different individual. The dotted line indicates the assay cut-off.