Fig. 3. 70AG mutation in RMRP causes a reduction in rRNA per cell.
a Example data from FlowFISH experiments, showing 18 S and 28 S rRNA detected by fluorescently-labeled antisense oligonucleotide probes, or signal from control probes with scrambled sequences. Gated on morphologically live cells. b Quantification of rRNA signals obtained in FlowFISH experiments. In each experiment, intensity values for each 70AG clone were normalized to the signal from wild-type cells. The gating strategy is shown in Supplementary Fig. 2c. The plot shows mean of data from three independent experiments, with SD. c Ratio of 28 S to 18 S rRNA in WT and 70AG cells, as determined by BioAnalyzer analysis. Mean and SD of data obtained in three independent experiments (total of 4 WT and 9 mutant samples). Nonsignificance concluded from two-tailed unpaired t-test (p = 0.8963, t = 0.1334, df=11). Source data are provided as a Source Data file.