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Selection of reaction temperature and time for detecting DuCV by RF-RAA. (A–F) shows the fluorescence curves under the reaction conditions of 25 min, 37–42°C. According to the peak value and growth rate of fluorescence curve, 41°C and 15 min were determined as the best reaction conditions. Abbreviations: DuCV, duck circovirus; RF-RAA, real-time fluorescence-based recombinase-aided amplification.
