Table 1.

Primers selected for DuCV reaction by RF-RAA.

Primers	Sequence (5′-3′)	Gene localization
DuCV-1-F	CCTCTGATCTGGCCGAAGCGA CATCCGCTGT	377-407
DuCV-2-F	CTGTGATGGCTGGCGTCCCGC TGACTGAGGTG	404-435
DuCV-3-F	CCGCTGACTGAGGTGGCCCGG AAGTTCCCC	421-450
DuCV-1-R	TGCCGGGAGGACCAATCAG AACGATGACTT	530-557
DuCV-2-R	AATTCAAATGCATAACGGC TCTTTCCGGTG	558-587
DuCV-3-R	CCGCGTGGTTTGTAATACTTGT TTTCGGCG	591-620

Abbreviations: DuCV, duck circovirus; RF-RAA, real-time fluorescence-based recombinase-aided amplification.