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. 2022 Jan 1;298(2):101559. doi: 10.1016/j.jbc.2021.101559

Figure 2.

Figure 2

A/B compartment switches during spermatogonial differentiation.A, Pearson correlation analysis illustrating the correlation of A/B indices between Un-SG and Di-SG samples. B, principal component analysis plot showing the A/B index profiles of Un-SG and Di-SG samples. C, the proportions and lengths of A/B compartments in genome. D, left, saddle plot showing the compartment strength in chromosome 9. Right, the compartment strength in all Un-SG and Di-SG samples, defined as the A-A and B-B compartment interaction strength relative to the A-B compartment interaction strength. P: Mann-Whitney U test, one-tailed. E, the interaction strength between A-A, B-B, or A-B compartments. The data are presented as the mean ± SD of eight biological samples. P: Mann-Whitney U test, one-tailed. F, the numbers (upper panel) and proportions (lower panel) of genes in A/B compartments. G, left, the average expression levels of genes in A/B compartments in Un-SG or Di-SG. P: Mann-Whitney U test, one-tailed. Right, PCA1 (the first eigenvalues, the upper part) and RefSeq view (the middle part) of chromosome 15, 118020000 to 120020000, as well as RNA-seq coverage track of TNP1 (chromosome 15, 119037496–119038105, the lower part) showing that TNP1, which was upregulated during spermatogonial differentiation, was located in the B compartment in Un-SG but switched to the A compartment in Di-SG. Principal component analysis 1 was calculated via eigenvector decomposition on the observed/expected intrachromosomal interaction matrices. H, a schematic overview illustrating the proportions of genomic regions subjected to A/B compartment switches (A to B or B to A) between Un-SG and Di-SG. I, the average expression levels of genes that changed from A to B or from B to A. P: Mann-Whitney U test, one-tailed. J, the numbers of genes that changed from A to B or from B to A. K and L, gene ontology-biological process (GO-BP) analysis of genes that changed from A to B (K) or from B to A (L). Di-SG, differentiating spermatogonia; PCA, principal component analysis; Un-SG, undifferentiated spermatogonia.