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. 2022 Jan 21;9:804032. doi: 10.3389/fcell.2021.804032

FIGURE 2.

FIGURE 2

Quantitative effects of TFBS mutations in the bra proximal enhancer. (A) Comparison of normalized dual reporter intensity ratios for the wildtype and mutant bra reporter constructs. Each datapoint represents a different imaged embryo. Each color indicates embryos from the same replicate. The box plots indicate the outlier-adjusted minimum, 1st quartile, median, 3rd quartile and outlier-adjusted maximum for each construct. Outliers are marked as lozenges. Asterisks indicate differential reporter expression between the TFBS mutation construct and the wildtype construct. ***p < 0.001. (B–C) Representative sum-projected confocal images. (B), wild type reporter construct. (C), ZIC mutant reporter construct. Magenta: YFP reporter expression. Green: Bra>H2B:HA internal control expression. Gray, phalloidin. Scale bar, 20 µm.