Skip to main content
. 2022 Jan 21;9:804032. doi: 10.3389/fcell.2021.804032

FIGURE 5.

FIGURE 5

Analysis of reporter expression in primary versus secondary notochord. (A) Left: normalized reporter expression in primary notochord. Right: normalized reporter expression in secondary notochord. Asterisks indicate differential reporter expression between the mutant construct and the wildtype construct. ***p < 0.001. (B) Schematic indicating how the primary/secondary normalized expression ratio was calculated. (C) The ratio of normalized primary notochord reporter expression to normalized secondary notochord reporter expression. Asterisks indicate a differential primary/secondary ratio between the mutant construct and the wildtype construct. ***p < 0.001. **p < 0.01. *p < 0.05. (D,E) Representative sum-projected confocal images. (D): wild type. (E): RBPJ. Magenta: YFP reporter expression. Green: Bra>H2B: HA internal control expression. Gray: phalloidin. Scale bar, 20 µm.