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. 2022 Jan 21;10:803559. doi: 10.3389/fbioe.2022.803559

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Copyright © 2022 Tuck, Leinecker, Watkin, Somers, Forsyth and Machuca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IMARIS (Bitplane) analysis of replicate Nyquist CLSM micrographs as % live cells and % dead cells, and mean signal intensity from green and red channels (left). Fluorescent pixels (pixels that returned a green or red signal) divided by the total volume of data provides an estimation of biofilm compactness (right). This data was constructed from micrograph compactness values before (T₀) and after 4 h of exposure to CTA-4OHcinn.