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. 2022 Jan 21;10:803559. doi: 10.3389/fbioe.2022.803559

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Copyright © 2022 Tuck, Leinecker, Watkin, Somers, Forsyth and Machuca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CLSM micrographs depicting K. pneumoniae cells stained with a 10 x concentration of Cellbrite Fix 555; where (A–C) = untreated control sample and (D–F) = CTA-4OHcinn treated sample. (A,D) compare combined channels, (B,E) compare transmission detector channels and (C,F) compare fluorescent signal only. The white arrow indicates a live cell with low Cellbrite Fix 555 signal (membrane fluorescence) and the black arrow indicates a membrane compromised cell emitting greater signal.