Table 2.
Characterization of ceramides present in cells and exosomes from GCS phenotypes using UHPLC/ESI(−)-Q-TOF-MS/MS a .
| LCB-related ions—common ions for (dLCB d18:1): m/z 263.2378 and m/z 237.2232 | |||||||
|---|---|---|---|---|---|---|---|
| Ceramide | Ceramide common ions b | FA most significant related ions b | Shorthand notations | ||||
| Cer | Rt(min) | Monoisotopic mass | [M+HCOOH-H]− | [M-H]− | [M-H-256]− | Type | Abbrev chains |
| C14 Cer | 11.2 | 509.4808 | 554.4781 | 508.4730 | 252.2333 | C14:0 | Cer 18:1;O2/14:0 |
| C14:1 Cer | 10.3 | 507.4594 | 552.4631 | 506.4573 | 250.2132 | C14:1 | Cer 18:1;O2/14:1 |
| C16 Cer | 13.1 | 537.5121 | 582.5106 | 536.5044 | 280.2646 | C16:0 | Cer 18:1;O2/16:0 |
| C16:1 Cer | 12.3 | 535.4962 | 534.4894 | 534.4888 | 278.2493 | C16:1 | Cer 18:1;O2/16:1 |
| C18 Cer | 14.8 | 565.5434 | 610.5400 | 564.5358 | 308.2960 | C18:0 | Cer 18:1;O2/18:0 |
| C18:1 Cer | 13.7 | 563.5277 | 608.5254 | 562.5200 | 306.2800 | C18:1 | Cer 18:1;O2/18:1 |
| C20 Cer | 16.5 | 593.5747 | 638.5709 | 592.5670 | 336.3275 | C20:0 | Cer 18:1;O2/20:0 |
| C20:1 Cer | 15.4 | 591.5521 | 636.5570 | 590.5515 | 334.3111 | C20:1 | Cer 18:1;O2/20:1 |
| C22 Cer | 18.1 | 621.6060 | 666.6025 | 620.5983 | 364.3579 | C22:0 | Cer 18:1;O2/22:0 |
| C22:1 Cer | 17.5 | 619.5879 | 664.5866 | 618.5835 | 362.3432 | C22:1 | Cer 18:1;O2/22:1 |
| C24 Cer | 19.4 | 649.6373 | 694.6339 | 648.6306 | 392.3898 | C24:0 | Cer 18:1;O2/24:0 |
| C24:1 Cer | 18.3 | 647.6216 | 692.6199 | 646.6128 | 390.3736 | C24:1 | Cer 18:1;O2/24:1 |
| C26:1 Cer | 19.6 | 675.6529 | 720.6487 | 674.64563 | 418.4050 | C26:1 | Cer 18:1;O2/26:1 |
a
The m/z value for the base peak are given in bold type.
b
For complete characterization and details, see reference (48).