FIGURE 2.

(A) Confocal images of C2C12 cells cultured in differentiation medium transfected with scramble (control) siRNA (left) or MALAT1 siRNA (right) showing impaired differentiation with MALAT1 silencing. (B) Expression levels of miR-34a-5p in C2C12 mouse myoblasts transfected with scramble control or with MALAT1 siRNA (MALATsi). siRNA transfection is effective after 72 h, and shows increased miR-34a-5p levels with MALAT1 inhibition. (C) Decreased expression of MALAT1 in C2C12 mouse myoblasts after exposure to hydrogen peroxide (H₂O₂). (D) The decrease in MALAT1 expression after hydrogen peroxide exposure is attenuated with p53 inhibition using siRNA (p53si). (E) Expression levels of TGF-β1 in C2C12 mouse myoblasts transfected with scramble control or with MALAT1 siRNA (MALATsi). siRNA transfection is effective after 72 h, and shows increased TGF-β1 levels with MALAT1 inhibition. ns P > 0.05, ^**P < 0.01, ^***P < 0.001. All assays were performed in triplicate.