Figure 1.

The establishment of the severe acute respiratory syndrome coronavirus (SARS-CoV-2) infected pregnant mice model. (A) The protocol of experiments. (B) The mRNA-relative quantitation of SARS-CoV-2 N expression in pulmonary tissues showed the difference in virus load between non-pregnant and pregnant during 4–7 days post-infection (dpi). CTR, control. (C) The pathological changes of the infected lung in hematoxylin and eosin (HE). (D–G) Weight changes. After being infected by virus or mock, the weights of the mice were recorded up to 7 dpi. Data were expressed as a percent of change from the baseline body weight (X ± SE, D) n = 13 for infected unpregnant, n = 25 for mock control; (E) n = 5 for infected (Early pregnant, started from E3.5 ± 1), n = 5 for pregnant control; (F) n = 8 for infected (Early to middle pregnant, started from E7.5 ± 1), n = 5 for pregnant control; and (G) n = 6 for infected (middle to late pregnant, started from E12.5 ± 1), n = 6 for pregnant control. Two-way ANOVA was used for time-related curve data here, while the Unpaired t-test or Mann-Whitney test was used for two groups compare. ns p > 0.05, ^***p < 0.001.