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. 2022 Jan 21;12:752934. doi: 10.3389/fphar.2021.752934

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Copyright © 2022 Tian, Welte, Mai, Liu, Ramirez and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Partition of aptamer in circulation. 4T1 tumor-bearing mice were treated i.v. with 0.4 nmol Cy5-T1 thioaptamer (T1) or Cy5-scramble aptamer (Scr), and periphery blood samples were collected 30 min and 4 h later for fluorescent analysis. (A) Fluorescent intensity in serum and PBMCs. ND: not detectable. (B) Flow cytometry analysis of cell-associated Cy5-aptamers at the 30 min and 4 h time points. (C) Distribution of Cy5-aptamer among cell subsets in PBMC. MFI: median fluorescence intensity.