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High MDSC-binding capacity from the M1 aptamer (A) tSNE map of T1 and M1 binding to PBMCs ex vivo. (B) Quantitative analysis of PBMC binding by aptamers. MFI: median fluorescence intensity. (C) Images of 4T1 spheroids with cell-transported aptamers. Upper panels: spheroids co-cultured with PBMCs pre-incubated with Cy5-aptamer. Bottom panels: spheroids co-cultured with free Cy5-aptamer. Green dots: CFSE-labeled PBMCs; red dots: Cy5-aptamer. Scale bar: 100 mm.
