Figure 7.

Sal suppressed pyroptosis in Aβ1-42 and D-gal/AlCl₃-induced AD mice via NLRP3/Caspase-1 or TLR4/NF-κB/NLRP3/Caspase-1 signaling pathway. (A,B) Western blot analysis of TLR4, MyD88, p-NF-κB, NF-κB, NLRP3, ASC, and cleaved caspase-1. p-NF-κB was normalized with NF-κB and others were normalized with β-actin. (C) Immunochemical staining of TLR4 in the CA1, CA3, and DG regions of the hippocampus of AD mice brains, and the IHC staining results are shown by arrows; scale bar = 50 μm, Original magnification: x200. Quantitative analyses of the TLR4 positive area in the hippocampus by Image J. Values are expressed as mean ± SEM (n = 3). ^##P < 0.01, ^###P < 0.001, vs. control group, ^*P < 0.05, ^**P < 0.01, ^***P < 0.01 vs. Aβ1-42 and D-gal/AlCl₃ group.