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. 2022 Jan 5;298(2):101561. doi: 10.1016/j.jbc.2022.101561

Figure 3.

Figure 3

Lack of Nogo expression promotes healthy expansion of adipose tissue by inhibiting fibrosis and collagen synthesis. The following assays were conducted for eWAT and sc-WAT tissues collected from mice used in Figure 2. eWAT sections were conducted H&E staining (A) with quantitation of adipocyte size (B, n = 6) and Sirius red staining (G, n = 5). C–F, expression of adiponectin, leptin, Vegf-β, and Col6α2 mRNA in eWAT was determined by qRT–PCR (n = 6). sc-WAT sections of HFD-fed littermate control and Nogo−/− mice (it is hardly to collect sc-WAT when the animals were fed normal chow, Fig. 2H) were conducted H&E staining (H) with quantitation of adipocyte size (I, n = 6) and Sirius red staining (N, n = 5). J–M, expression of adiponectin, leptin, Vegf-β, and Col6α1 mRNA in sc-WAT was determined by qRT–PCR (n = 6). #p < 0.05, ##p < 0.01, ###p < 0.001 versus control HFD-fed mice. Col6α1, collagen type VI α1; Col6α2, collagen type VI α2; eWAT, epididymal white adipose tissue; HFD, high-fat diet; NC, normal chow; Nogo, reticulon-4; qRT, quantitative RT; sc-WAT, subcutaneous white adipose tissue; Vegf-β, vascular endothelial growth factor β.