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. 2022 Jan 5;298(2):101561. doi: 10.1016/j.jbc.2022.101561

Figure 6.

Figure 6

Nogo deficiency attenuates obesity-induced systemic inflammation. The following assays were conducted on the serum and tissues collected from mice used in Figure 2. A, serum inflammatory cytokines were determined by the mouse cytokine array G3 (RayBiotech) method (n = 2). B, H&E staining was conducted on the eWAT sections, and the number of crown-like structure (CLS) was determined. C–E, immunohistochemical staining was conducted on the liver, BAT, and islet sections to determine MOMA2 expression with quantitative analysis of MOMA2-positive cells (n = 6). Fractions of M1 and M2 macrophages were determined on eWAT (F and G) and BAT (H and I) by FACS (n = 4). #p < 0.05, ##p < 0.01, ###p < 0.001 versus control HFD-fed mice. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; FACS, fluorescence-activated cell sorting; HFD, high-fat diet; NC, normal chow; Nogo, reticulon-4.