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. 2022 Jan 5;298(2):101561. doi: 10.1016/j.jbc.2022.101561

Figure 8.

Figure 8

Nogo deficiency promotes NF-κB p65 degradation. A and B, protein expression of Nogo-B and NF-κB p65 was determined in 293T cells transfected with C2 vector or C2-Nogo-B expression vector (A, left panel) and Ctrl-Cas9 or Nogo-Cas9 HepG2 cells (A, right panel) by Western blot with quantitative analysis of band density (A), and expression of NF-κB p65 mRNA by qRT–PCR (B). C–E, Ctrl-Cas9, Nogo-Cas9 HepG2 cells (C and E), or 293T cells transfected with C2 vector or C2-Nogo-B expression vector (D) received the following treatment: cycloheximide (CHX, 100 μg/ml, C), MG132 (20 μmol/l, D), or NH4Cl (20 mmol/l, E) for indicated time. After treatment, total proteins were extracted and used to determine protein expression of NF-κB p65 and Nogo-B by Western blot with quantitative analysis of band density (n = 3). &p < 0.05 versus control cells. Nogo, reticulon-4; qRT, quantitative RT.