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. 2022 Feb 3;139(5):779–791. doi: 10.1182/blood.2021010762

Figure 4.

Figure 4.

CLPB mutants show impaired ATPase and disaggregase activity and exhibit a dominant-negative effect on WT CLPB. Purified CLPB protein was used to measure ATPase activity of the indicated CLPB variants tested in isolation (A); disaggregase activity of the indicated CLPB variants tested in isolation (B); ATPase activity of WT CLPB when mixed 1:1 with WT CLPB or CLPB variants (C); and disaggregase activity of WT CLPB when mixed 1:1 with WT CLPB or CLPB variants (D). In (C-D), the dotted line represents 50% of WT CLPB activity. Data represent 3 independent experiments. *P < .05, **P < .01, ***P < .001, ****P < .0001, 1-way ANOVA. In (A-B), comparison was made with WT CLPB activity. A comparison was made with values representing 50% of WT activity (C-D).