Table 2.

LC gradient elution program for untargeted lipidomic analysis

Time (min)	Flow rate (mL/min)	Gradient (% B)
0.0	0.3	30
2.0	0.3	52
3.5	0.3	63
5.0	0.3	68
6.0	0.3	64
12.5	0.3	74.5
13.5	0.3	80
14.5	0.3	30
19.0	0.3	30