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. 2022 Jan 20;23(3):231. doi: 10.3892/etm.2022.11155

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Copyright: © Shi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of TRAF6 abolishes the effects of miR-146b-5p in nude mice. (A) HT29 cells transfected with GFP-TRAF6 overexpression or GFP control vector cells subjected to western blotting analysis. (B) HT29 cells stably overexpressing GFP-TRAF6 or not were injected into nude mice. Tumor samples are presented. (C) Expression of miR-146b-5p was determined using reverse transcription-quantitative PCR. (D) Weights of xenograft tumors. (E) Volumes of xenograft tumors. n=3. ^*P<0.05 and ^**P<0.01 vs. NC. ^#P<0.05 and ^##P<0.01 vs. miR-146b-5p mimics. miR, microRNA; TRAF6, tumor necrosis factor receptor-associated factor 6; NC, negative control; GFP, green fluorescence protein; n.s. not significant.