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. 2022 Jan 18;14(4):5066–5079. doi: 10.1021/acsami.1c22434

Figure 2.

Figure 2

Specific accumulation of LPNP-Au-555 in microglial cells in the rat hypothalamus. (A1–A3) Colocalization of LPNP-Au-555 (red) and ionized calcium-binding adaptor molecule 1 immunoreactivity (Iba1-ir, green) in microglial cells. The dotted lines in panels (A1, A2) show the diffusion of LPNP-Au-555 at a radius of ≈300 μm around the injection spot (asterisk in panels A1, A2). An arrowhead in panel (A1) shows an Iba1-ir microglial cell that does not contain LPNP-Au. (A4) NeuroTrace histochemical staining (blue) in the same area of panels (A1–A3). (B1–B4) High-magnification images of the area framed in panels (A1, A2, A4). Arrows in panel (B1) show Iba1-ir microglia containing LPNP-Au-555. Microglia located at the edge of the area diffused by LPNP-Au-555 are indicated by arrowheads in panel (B1). Panels (B3, B4) show no accumulation of LPNP-Au-555 in the NeuroTrace-stained neurons. (C1–C4) No accumulation of LPNP-Au-555 is observed in glial fibrillary acidic protein-ir (GFAP-ir) astrocytes (cyan). Arrows in panel (C1) show microglia containing both LPNP-Au-555 and Iba1-ir. n = 4. Scale bar: 100 μm in panels (A1–A4) and 20 μm in panels (B1–B4) and (C1–C4).