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. Author manuscript; available in PMC: 2022 Feb 4.
Published in final edited form as: J Vis Exp. 2020 Jun 8;(160):10.3791/61060. doi: 10.3791/61060

Materials.

Name Company Catalog Number Comments
2100 Bioanalyzer Agilent G2939BA
Agilent DNA 7500 Kit Agilent 5067–1506
Agilent High Sensitivity DNA Kit Agilent 5067–4626
Agilent RNA 6000 Nano Kit Agilent 5067–1511
AllPrep DNA/RNA FFPE Kit Qiagen 80234
CFX96 Touch System Bio-Rad 1855195
Library Quantification kit v2-Illumina KapaBiosystems KK4824
NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs E7765S https://www.neb.com/protocols/2017/02/07/protocol-for-use-with-ffpe-rna-nebnext-rrna-depletion-kit
NEBNext rRNA Depletion Kit (Human/Mouse/Rat) New England Biolabs E6310L
NextSeq 500 Sequencing System Illumina SY-415–1001 NextSeq 500 System guide: https://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/nextseq/nextseq-500-system-guide-15046563-06.pdf
NextSeq PhiX Control Kit Illumina FC-110–3002
NSQ 500/550 Hi Output KT v2.5 (150 CYS) Illumina 20024907
10X Genomics Magnetic Separator 10X Genomics 120250
Rotator Multimixer VWR 13916–822
C1000 Touch Thermal Cycler Bio-Rad 1851197
Sequencing reagent kit Illumina 20024907
Flow cell package Illumina 20024907
Buffer cartridge and the reagent cartridge Illumina 20024907
Sodium hydroxide solution (0.2N) Millipore Sigma SX0607D-6
TRIS-HCL Buffer 1.0M, pH 7.0 Fisher Scientific 50–151–871