Gel image generated by the Agilent 2100 bioanalyzer of amplified vanA, vanB, vanC1, vanC2/C3, E. faecalis-specific, E. faecium-specific, and rrs genes by the optimized multiplex PCR assay (1-2) containing a novel primer combination as described above. Lanes: M, DNA ladder; 1, an E. faecalis vanA isolate; 2, an E. faecalis vanA isolate; 3, an E. faecalis vanB isolate; 4, an E. faecalis vanB isolate; 5, an E. faecium vanA isolate; 6, an E. faecium vanA isolate; 7, an E. faecium vanB isolate; 8, an E. faecium vanB isolate; 9, an E. gallinarum vanC1 isolate; 10, an E. gallinarum vanC1 and vanA isolate; 11, an E. gallinarum vanC1 and vanB isolate; 12, an E. casseliflavus or E. flavescens vanC2 or vanC3 isolate.