Figure 2.

In vivo imaging studies with Luc mRNA-LNPs

Frozen Luc mRNA-LNPs were stored at −80°C throughout the study, and lyophilized Luc mRNA-LNPs were stored at −80°C, −20°C, 4°C, 25°C, or 42°C for 0, 4, or 24 weeks prior to reconstitution. (A–H) Mice were (A–D) IM- or (E–H) ID-injected with 3 μg Luc mRNA-LNPs (frozen or reconstituted lyophilized products), and bioluminescence was monitored for 9 days. (A) Representative IVIS images taken 2 days post-IM Luc mRNA-LNP injection. (B–D) Quantification of the bioluminescent signal obtained after IM injection of frozen and reconstituted lyophilized Luc mRNA-LNPs after (B) 0, (C) 4- or (D) 24-week storage. (E) Representative IVIS images taken 2 days post-ID Luc mRNA-LNP injection. (F–H) Quantification of the bioluminescent signal obtained after the ID injection of frozen and reconstituted lyophilized Luc mRNA-LNPs after (F) 0, (G) 4-, or (H) 24-week storage. n = 5 mice per group. Error bars are SEM. See also Figures S6 and S7.