When macrophages from WT mice are primed with LPS, and stimulated with soluble stimuli such as ATP, Lyn is activated, and then phosphorylates NLRP3 at Tyr 918 which is a prerequisite for NLRP3 ubiquitination and subsequent degradation in the proteasome. In the absence of Lyn, NLRP3 tyrosine phosphorylation and subsequent ubiquitination is abrogated, which results in heightened NLRP3 protein expression, and activation.