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. Author manuscript; available in PMC: 2022 Feb 4.
Published in final edited form as: Biochem J. 2009 Jun 12;421(1):107–118. doi: 10.1042/BJ20090185

Figure 2.

Figure 2.

A. UV-crosslinking to detect specific binding of PfPRMT1 to AdoMet. Either recombinant MBP-PfPRMT1 (upper bands) or PfPRMT1 (lower band) was used in the binding reactions. 1000-fold excess of unlabeled ATP or AdoMet was included as the competitor. Upper panel shows Coomassie blue-stained gel, and lower panel shows the fluorograph. B. Oligomerization of recombinant PfPRMT1. Recombinant PfPRMT1 was incubated without (lane 1) or with 0.025% glutaraldehyde for 3 min (lane 2) and 5 min (lane 3), respectively. After cross-linking the proteins were separated in 10% SDS-PAGE and probed with the anti-PfPRMT1 antiserum. Monomers, dimers, tetramers, and multimers are indicated. The asterisk indicates a bacterial protein present in the purified PfPRMT1, which does not show detectable crosslinking under these conditions.