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. Author manuscript; available in PMC: 2022 Feb 4.
Published in final edited form as: Biochem J. 2009 Jun 12;421(1):107–118. doi: 10.1042/BJ20090185

Figure 5.

Figure 5.

PfPRMT1 activity on potential substrates. Nine P. falciparum proteins were expressed in E. coli as either GST- or MBP-fusion proteins, purified, and incubated with MBP-PfPRMT1 in methylation assays. Upper panel shows the Coomassie blue stained gel, and lower panel is the fluorograph. GST-fusion proteins include PABPII (1-202), fibrillarin (1-100), putative PABP (1-383), Gar1-like (1-209), PF10_0068 (1-246), PFF1135w (128-347), and PF10_0217 (391-538). MBP-fusion proteins include PFE0865c (1-298), FE0865c-C (199-298), PFE0865c-N (1-112), and rpS2 (1-60).