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The results of Western-blot analysis and the relative expression of Piezo1, NLRP3, IL-1β, MMP9, MMP13, TGF-β, Aggrecan, and Col2α1 in the nucleus pulposus cells of the Low-impact group. A, B, The protein expression of Piezo1, NLRP3, IL-1β, MMP3, and MMP9 in NP cells increased significantly and time dependently, accompanied by significantly decreased expression of Aggrecan and Col2α1(∗P < 0.05 vs. Control group).
