Fig 6. Influence of the size of the fitted region.

Precision (solid lines) and CRB (dashed lines) are shown for fitted regions of interest ranging from 3 pixels to 41 pixels. Simulations were carried out for two different noise levels, b = 0 (pink lines) and b = 300 (black lines) and for three different defocus values. The inclination angle was set to θ = π/6. A list of all simulation parameters is given in S1 Table in S1 File.