Table 2.

Dilutions of Fmoc-OSu in ethanol used for Fmoc quantitative assay

Standard	Amount of standard to add (μL)	Source	Amount of ethanol to add (μL)	Final Concentration (mM)	Absorbance range
A	300	1 mM Stock	0	1	1.820 – 1.875
B	200	1 mM Stock	100	0.75	1.300 – 1.391
C	300	1 mM Stock	300	0.5	0.999 – 1.029
D	300	C	300	0.25	0.626 – 0.689
E	300	D	300	0.125	0.443 – 0.468
F	300	E	300	0.063	0.344 – 0.353
G	300	F	300	0.031	0.276 – 0.295
H	0	-	300	0	0.204 – 0.220