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. 2022 Jan 28;2022:6966470. doi: 10.1155/2022/6966470

Figure 3.

Figure 3

The effect of (a) pH of phosphate buffer solution, (b) incubation time of antibody and antigen, and (c) HCl concentration. (d) Calibration plot of CEA levels (the inset: UV-vis absorption spectrums in 400 nm–700 nm of colorimetric immunoassay toward different CEA concentrations).