Figure 8.

Immunohistochemical staining using 4HNE (marker for oxidative stress) antibodies in representative pancreatic sections of ADH⁻ (A1–E1; left) and ADH⁺ (A2–E2; right) deer mice, respectively. No positive staining for 4HNE was observed in the pancreas of pair-fed control diet and control diet plus FAEEs-fed ADH⁻ (A1 and B1) and ADH⁺ (A2 and B2) deer mice, respectively. A significant positive staining for 4HNE was observed in various chronic EtOH-fed groups of ADH⁻ vs. ADH⁺ deer mice, respectively. Pancreas of chronic EtOH-fed (C1), chronic plus binge EtOH-fed (D1), and chronic plus binge EtOH and FAEEs-fed (E1) ADH⁻ deer mice shows significant positive staining for 4HNE. However, the positive staining for 4HNE was not observed in the pancreas of various chronic EtOH-fed groups of ADH⁺ deer mice (C2–E2). Quantification of the intensity of immunohistochemical staining for 4HNE (F) in the pancreas of ADH⁻ and ADH⁺ deer mice. Inset shows area of higher magnification (×40). Data were analyzed using ANOVA, followed by Tukey’s multiple comparisons test, and presented as means ± SE (n = 5 replicates). *P ≤ 0.05 chronic EtOH-fed group and chronic plus binge EtOH-fed group vs. pair-fed control diet group. #P ≤ 0.05 chronic plus single binge EtOH and FAEEs-fed group vs. control diet plus FAEEs-fed group and †P ≤ 0.05 chronic plus binge EtOH and FAEEs-fed group vs. chronic EtOH-fed group, respectively. ADH⁻, alcohol dehydrogenase-deficient; FAEEs, fatty acid ethyl esters; 4HNE, 4-hydroxynonenal.