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. Author manuscript; available in PMC: 2022 Feb 5.
Published in final edited form as: Eur J Cell Biol. 2021 Nov 14;101(1):151185. doi: 10.1016/j.ejcb.2021.151185

Fig. 3.

Fig. 3.

CBD causes Parkin-dependent LC3 colocalization. A. HeLa cells were treated with 10 μM AO and then imaged for Venus-Parkin, Smac-MTS-RFP, and LC3-FFP every hour. Representative images at 0, 2, 6 and 12 h shown, B Same as (A) but with cells treated with 50 μM CBD, C. Quantification of Venus-Parkin colocalization to Smac-MTS-RFP using Pearson’s Correlation Coefficient (PCC) of cells from (A) and (B) along with cells treated with Vehicle control. Error bar represent SD, t-test comparing vehicle to drug treated represented as p-value < .005 = **, n = 3, D. Quantification of LC3-CFP colocalization to Smac-MTS-RFP using Pearson’s Correlation Coefficient (PCC) of cells from (A) and (B) along with cells treated with Vehicle control. Error bar represent SD, t-test comparing vehicle to drug treated represented as p-value < .005 = **, n = 3, E. Cells were treated for with 50 μM CBD, 10 μM AO or Vehicle, and imaged at 0 and 16 h for mitochondrial content as seen in (A). Smac-MTS-RFP intensity was calculated, and fold change of mitochondrial content was compared between 0 and 16 h. t-test comparing vehicle to drug treated represented as p-value < .005 = **, n = 10–11.